The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine

The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine

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(1) Background: CIC-DUX4 sarcoma is a rare mesenchymal small round cell tumor which belongs to rare cancers that occupy a significant percentage of cancer cases as a whole, despite each being rare.Importantly, each rare cancer type has different features, and thus there is a need to develop a model that mimics the features of each of these cancers.We evaluated the idea that the chicken chorioallantoic membrane assay (CAM), a convenient and versatile animal model, can Gravy Pot be established for the CIC-DUX4 sarcoma.(2) Methods: Patient-derived cell lines of CIC-DUX4 were applied.These cells were transplanted onto the CAM membrane and tumor formation was examined by H&E staining, immunohistochemistry and Western blotting.

The CAM tumor was transferred onto a fresh CAM and was also used to Yanagiba Knives form organoids.Retention of the fusion gene was examined.(3) Results: H&E staining as well as molecular characterization demonstrated the formation of the CIC-DUX4 tumor on the CAM membrane.Expression of cyclin D2 and ETV4 was identified.The CAM tumor was transferred to a fresh CAM to form the second-generation CAM tumor.

In addition, we were successful in forming tumor organoids using the CAM tumor.Retention of the fusion gene CIC-DUX4 in the CAM, second-generation CAM, and in the CAM-derived organoids was confirmed by RT-PCR.(4) Conclusions: The CAM assay provides a promising model for CIC-DUX4 sarcoma.